Metodologia molecular de identificação de proteínas Cry em bioinseticidas através de PCR (Polymerase Chain Reaction) / Molecular methodology of identification of Cry proteins in bioinsecticides through PCR (Polymerase Chain Reaction)

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Development Application of Polymerase Chain Reaction (PCR)

Polymerase chain reaction (PCR) is an in vitro technique to synthesize large quantities of a given DNA molecule that separates the DNA into two complementary strands, uses a peltier heat pump to quickly heat and cool the DNA and uses the Taq polymerase for the synthesis of DNA. Taq is a bacterium that lives by volcanic sulfer jets at the bottom of the ocean where the temperature is very high. F...

Reverse transcriptase-polymerase chain reaction (rt-PCR).

Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...

Polymerase Chain Reaction (PCR): A Short Review

was awarded the Nobel prize in Chemistry along with Michael Smith for his work on PCR2. The PCR is commonly carried out in a reaction volume of 10-200 μl in small reaction tubes (0.2-0.5 ml volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction. Many modern thermal cyclers make use of the Peltier effe...